Absolutely not! Unlike some other threshold temperature products, Blood Temp 10 do not require pre-conditioning.nJust activate Blood Temp 10 at any temperature above 10°C/50°F as you cannot squeeze the dye out of the blister if it is in solid form. You then simply need to adhere the Timestrip onto a pre-chilled blood bag, whose temperature will immediately solidify the dye and track any 10 degree temperature breaches.