Do I need to condition Duo prior to activation?

No. Unlike some other threshold temperature products, Duo indicators do not require pre-conditioning.
However, you need to activate above the highest threshold temperature, as you cannot squeeze the dye out of the blister if it is in solid form.
The temperature of Duo needs to be raised to 35°C/95°F to be able to activate both indicators and then dropped to the lower of the two STOP temperatures (8°C/46°F) to stop the progress of both liquid dyes. The fastest way to do this is by placing Duo in an environment much colder than the STOP temperature (8°C/46°F) for a short period of time.

adminDo I need to condition Duo prior to activation?